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p43
IMG-EK (pro-EMAPII)
Antibody43
Chiral Building Blocks
Custom Synthesis
ARS
CGX Library

IMG-EK (pro-EMAPII), Recombinant (E.coli)

 

Size
200ug : \ 275,000 (US $ 275)
1mg : \ 1,100,000 (US $ 1,100)

100mg이상 주문시 견적을 보내드립니다

Description
IMG-EK (pro-EMAPII), also known as p43, is a new cytokine that stimulates apoptosis in endothelial cells. IMG-EK (pro-EMAPII) is one of the three auxiliary components invariably associated with nine aminoacyl-tRNA synthetases as a multienzyme complex . IMG-EK (pro-EMAPII), recombinant , is a 34.3kDa polypeptide containing 312 amino acids, that induces cell death of endothelial cells in vitro (1) and demonstrates potent anti-angiogenic activity (2,3).

Formulation
IMG-EK (pro-EMAPII), recombinant, is supplied as a dried powder.

Optimal Biological Range
For most in vitro applications IMG-EK (pro-EMAPII) exerts its biological activity in the concentration range 1nM~1uM.

Reconstitution
Dried IMG-EK (pro-EMAPII) is soluble in water and most aqueous buffers and can be diluted into other buffered solutions and stored at -20°C for future use.

Source
Recombinant DNA expressed in E. coli.

Storage Temperature
Store dried IMG-EK (pro-EMAPII), desiccated at -20°C. Avoid exposure to frequent temperature changes. Store reconstituted IMG-EK (pro-EMAPII), in aliquots at -20°C. The performance of this product is guaranteed for at least six months from the date of purchase, if stored and handled properly.

 

Quality Control
 

Purity
>99% in SDS PAGE gel

Biological Activity
The ED50 for FGF, Basic, i.e., the concentration of factor that produces one-half the maximal response, is determined in a cytotoxicity bioassay using bovine aortal endothelial cells in DMEM with 10% FBS and MTT assay. The ED50 value obtained is reported on the Product Information Label affixed to this document.

 

References
 
  1. A Cofactor of tRNA Synthetase, p43, Is Secreted to Up-regulate Proinflammatory Genes. J. Biol. Chem., 276:23028-23033, 2001

  2. A Novel Anti-tumor Cytokine Contains an RNA Binding Motif Present in Aminoacyl-tRNA Synthetases. J. Biol. Chem., 275: 27062-27068, 2000

  3. Dose-dependent Biphasic Activity of tRNA Synthetase-associating factor, p43, in Angiogenesis. J. Biol. Chem., 277: 45243-45248, 2002

 

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